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The recombinant protein 8 gene of the fth1 vector (VIII-STS) was modified by introducing the 5′ (A – orange) and 3′ (B – blue) Illumina adaptors such that they flank the DNA insert (E). Reference DNA barcodes were introduced between the A adaptor and the first SfiI site (bc, see “ Pre-processing of sequence data” in Methods). Samples for deep sequencing are generated directly by PCR using the adaptors as PCR primers.

Journal: PLoS ONE

Article Title: Deep Panning: Steps towards Probing the IgOme

doi: 10.1371/journal.pone.0041469

Figure Lengend Snippet: The recombinant protein 8 gene of the fth1 vector (VIII-STS) was modified by introducing the 5′ (A – orange) and 3′ (B – blue) Illumina adaptors such that they flank the DNA insert (E). Reference DNA barcodes were introduced between the A adaptor and the first SfiI site (bc, see “ Pre-processing of sequence data” in Methods). Samples for deep sequencing are generated directly by PCR using the adaptors as PCR primers.

Article Snippet: As is illustrated in we modified the fth1 phage display vector to contain the Illumina adaptor sequences upstream and downstream to the SfiI cloning sites of the recombinant protein 8 gene thus generating fth1-dp.

Techniques: Recombinant, Plasmid Preparation, Modification, Sequencing, Generated